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Small angle X-ray study of cellulose macromolecules produced by tunicates and bacteria

last modified Jun 11, 2014 03:38 PM
A new paper on cellulose by Mudrika Khandelwal and Alan Windle was published in the International Journal of Biological Macromolecules.
Small angle X-ray study of cellulose macromolecules produced by tunicates and bacteria

SEM image of microfibrils

A study of the structure of cellulose microfibrils from three different sources (bacterial, nata de coco, and tunicate) published in the International Journal of Biological Macromolecules.

"The organisation of poly-glucan chains into cellulose macromolecular microfibrils has been studied using small angle X-ray scattering (SAXS). Three kinds of cellulose – bacterial cellulose (BC), nata-de-coco (NdC) (food grade bacterial cellulose) and tunicate cellulose (TC) have been investigated. Given the large ambiguity in literature on the microfibril dimensions owing to different methods and data analysis strategies, a method to extract dimensions of cellulose microfibrils using SAXS has been shown, which was found to be consistent across all the samples. The results have been verified with microscopy data. Two populations of microfibrils with different cross-section dimensions were identified. The dimensions of the rectangular cross-sections of BC were found to be 32 nm by 16 nm and 21 nm by 10 nm. The dimensions for NdC were calculated to be 25 nm × 8 nm and 14 nm × 6 nm and that for TC were determined to be 25 nm × 10 nm and 15 nm × 8 nm."

Link to article: http://dx.doi.org/10.1016/j.ijbiomac.2014.05.007

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